Wool has a long history of use in textiles throughout human civilization. Many smart functions such as reversible shape changes to various stimuli have been demonstrated in the last few years. However, the force-related characteristics are still imperfectly recognized, although they are expected to be used as actuators due to their biological origins and broad applications. Herein, we investigated the feasibility of wools in performing actuating ability through its intrinsic structures and fabrication methods. The diverse modes of contractive forces were obtained in wool materials including platform-like, double-peak, and slope-like shapes, where a molecular model was also presented to trace the origins of stress evolution. After that, a polymeric blend was created to modify the wool materials and a dissimilar performance of stress production was achieved, a square stress mode with stable manner and maintenance, for broad applications in a more efficient way. It is believed that these actuating properties extracted from natural hairs have a large potential in current smart applications and lay down new inspiration in designing actuators.
Rachael N. Parker, Dana M. Cairns, Wenyao A. Wu, Kathryn E. Jordan Ā· 8 authors
Three-dimensional organoid tissue culture models are a promising approach for the study of biological processes including diseases. Advances in these tissue culture technologies improve in vitro analysis compared to standard 2D cellular approaches and are more representative of the physiological environment. However, a major challenge associated with organoid systems stems from the laborious processing involved in the analysis of large numbers of organoids. Here the design, characterization, and application of silk-elastin-like protein-based smart carrier arrays for processing organoids is presented. Fabrication of hydrogel-based carrier systems at room temperature result in organized arrays of organoids that maintain tissue culture plate orientation and could be processed simultaneously for histology. The system works by transfer of the organoids to the hydrogel arrays after which the material is subjected to 65 Ā°C to induce hydrogel contraction to secure the organoids, resulting in multisample constructs and allowing for placement on a microscope slide. Histological processing and immunostaining of these arrayed cerebral organoids analyzed within the contracted silk-elastin-like proteins (SELP) show retention of native organoid features compared to controls without the hydrogel carrier system, thus avoiding any artifacts. These SELP carriers present a useful approach for improving efficiency of scaled organoid screening and processing.